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Description
Research Area
Neuroscience
Images & Validation
−Item 1 of 2
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:8000, IHC-P - 1:25 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Monkey |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | BTD55833 |
| Immunogen | This TUBA4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 417-446 amino acids from the C-terminal region of human TUBA4A. Antigen Region: 417-446 aa. |
| Target | TUBA4A |
| Molecular Weight | 49924 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain, TUBA4A, TUBA1
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All lanes: Anti-TUBA4A Antibody (C-term) at 1:8000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: Mouse brain lysate. Lane 7: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Staining TUBA4A in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001265481.1, NP_005991.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
TUBA4A Antibody (C-term) (orb1936611)
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