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UNG Active

SKU: orb1994998

Description

Recombinant E.Coli Uracil DNA Glycosylase, Active

Images & Validation

Application Notes
Applications: Treatment of 0.1μg of uracil containing DNA with 1U UDG for 10 min. at 37℃ renders the DNA incapable of being copied by DNA polymerase. The enzyme can be 95% heat killed by incubation at 95℃ for 10 minutes. Since UDG remains partially active following heat treatment at 95℃, it is recommended that uracil glycosylase inhibitor be added to prevent degradation of product DNA. Alternatively, reaction products can be immediately extracted with phenol/chloroform. Unit Definition: 1 unit is defined as the amount of enzyme that catalyzes the release of 60pmol of uracil/minute from double-stranded, uracil-containing DNA. Activity is measured by release of [3H]-uracil in a 50µl reaction containing 0.2µg DNA (104-105 cpm/µg) in 30 min. at 37°C

Key Properties

SourceEscherichia Coli
PurityGreater than 97.0% as determined by SDS-PAGE.

Storage & Handling

StorageStability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles
Form/AppearanceSterile Filtered clear solution.
Buffer/PreservativesUNG protein solution (5U/ul) containing 10mM Tris-HCl (25℃, pH 7.4), 50mM KCl, 0.1 mM EDTA, 1mM DTT, 0.1mg/ml rAlbumin & 50% glycerol.
DisclaimerFor research use only

Alternative Names

UDG, b2580, JW2564, EC 3.2.2.27, DGU, UNG15, HIGM5, Uracil-DNA Glycosylase 1, EC 3.2.2, HIGM4, UNG2.

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UNG Active (orb1994998)

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Available Sizes

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1000 U
$ 260.00
5000 U
$ 550.00
50000 U
$ 3,350.00
DispatchUsually dispatched within 5-10 working days
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