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VASP (phospho Ser157) rabbit pAb Antibody
SKU: orb770356
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Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human VASP (phospho Ser157) |
| Molecular Weight | 39 46 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-VASP antibody, anti-Vasodilator-stimulated phosphoprotein antibody, anti-VASP antibody
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Immunohistochemical analysis of paraffin-embedded Human tonsil. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemistry analysis of paraffin-embedded human breast cancer, using VASP (Phospho-Ser157) Antibody. The picture on the right is blocked with the VASP (Phospho-Ser157) peptide.
Western blot analysis of VASP (Phospho-Ser157) Antibody. The lane on the right is blocked with the VASP (Phospho-Ser157) peptide.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
VASP (phospho Ser157) rabbit pAb Antibody (orb770356)
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