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Recombinant VIM Antibody / Vimentin
SKU: orb1151550
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | Mouse IgG1, kappa |
| Clone No. | rVIM/6431 |
| Immunogen | A portion of amino acids 2-466 from the human protein was used as the immunogen for the recombinant VIM antibody. |
| Purification | Protein A/G affinity |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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IHC staining of FFPE human colon tissue with recombinant VIM antibody (clone rVIM/6431). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant VIM antibody (clone rVIM/6431). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human U-87 MG cell lysate using recombinant VIM antibody (clone rVIM/6431). Predicted molecular weight ~53 kDa.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
Recombinant VIM Antibody / Vimentin (orb1151550)
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