Recombinant Wilms Tumor 1 Antibody

SKU: orb2642959

Description

Wilms Tumor is a transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. [UniProt]

Research Area

Cancer Biology

Images & Validation

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Item 1 of 1

Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
Reactivity	Human
Application Notes	Titering of the recombinant Wilms Tumor 1 antibody may be required for optimal performance.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	MMWT1-1
Immunogen	A recombinant human protein was used as the immunogen for the recombinant Wilms Tumor 1 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC testing of FFPE human mesothelioma with recombinant Wilms Tumor 1 antibody (clone MMWT1-1). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Quick Database Links

UniProt

P19544

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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