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XRCC5 Recombinant Mouse Monoclonal Antibody
SKU: orb1172974
Description
Research Area
DNA Repair, DNA and RNA
Images & Validation
−Item 1 of 2
| Tested Applications | ICC, WB |
|---|---|
| Dilution Range | WB=1:500-2000, ICC/IF=1:50-200 |
| Reactivity | Human |
| Predicted Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG1, Kappa |
| Clone No. | F1D10 |
| Target | XRCC5 |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 4°C overnight, Lysate: HeLa, Jurkat, A549, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 86 kDa, Observed MW: 86 kDa.
Cell line: HeLa, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1172974.
Quick Database Links
Gene Symbol
XRCC5
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
XRCC5 Recombinant Mouse Monoclonal Antibody (orb1172974)
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