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ZAP70 Antibody
SKU: orb2641394
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, FACS, IF, IHC-P |
|---|---|
| Dilution Range | Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | ZAP70/2035 |
| Immunogen | A portion of amino acids 247-382 from the human protein was used as the immunogen for the ZAP70 antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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ZAP70 Antibody [orb1410176]
ELISA, IHC, WB
Human
Rabbit
Monoclonal
Unconjugated
20 μg, 100 μg, 100 μg (without BSA and Azide)
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IHC testing of FFPE human tonsil tissue with ZAP70 antibody (clone ZAP70/2035). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of PFA-fixed human Jurkat cells with ZAP70 antibody (green, clone ZAP70/2035) and Reddot nuclear stain (red).
Flow testing of PFA-fixed Jurkat cells with ZAP70 antibody (clone ZAP70/2035); Red = isotype control, Blue = ZAP70 antibody.
Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using ZAP70 antibody (clone ZAP70/2035). These results demonstrate the foremost specificity of the ZAP70/2035 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free ZAP70 antibody (clone ZAP70/2035) as confirmation of integrity and purity.
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ZAP70 Antibody (orb2641394)
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