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14-3-3 Recombinant Mouse Monoclonal Antibody
SKU: orb1172936
Description
Research Area
Alzheimer's Disease, Cancer Research, Cell Biology, Cytoplasmic, Neurology, Neuroscience, Signal Transduction, Synapse Markers, Tumor Biology
Images & Validation
−Item 1 of 2
| Tested Applications | ICC, WB |
|---|---|
| Dilution Range | WB=1:500-2000, ICC/IF=1:50-200 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG1, Kappa |
| Clone No. | B12C1 |
| Target | YWHAZ |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−pan 14-3-3; 14 3 3 protein beta; 14 3 3 protein beta/alpha; 14 3 3 protein zeta; KCIP 1; Protein 1054; Protein kinase C inhibitor protein 1; 14 3 3 protein delta; 14 3 3 protein gamma; YWHAB; YWHAZ; 14 3 3 protein Epsilon.
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Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: HeLa, 2: A549, 3: Neuro-2a, 4: PC-12, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 28 kDa, Observed MW: 29 kDa.
Cell line: HepG2, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% Triton X-100, Primary Ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172936.
Quick Database Links
Gene Symbol
YWHAZ
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
14-3-3 Recombinant Mouse Monoclonal Antibody (orb1172936)
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