14-3-3/YWHAZ Mouse Monoclonal Antibody

SKU: orb500615

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Description

Research Area

Alzheimer's Disease, Cancer Research, Cell Biology, Cytoplasmic, Neurology, Neuroscience, Signal Transduction, Synapse Markers, Tumor Biology

Images & Validation

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Item 1 of 2

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone No.	1B6
Immunogen	Recombinant human YWHAZ protein
Target	YWHAZ
Molecular Weight	27 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Zeta; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, delta Polypeptide; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Zeta Polypeptide; Protein Kinase C Inhibitor Protein 1; 14-3-3 delta; 14-3-3 Zeta; KCIP-1; Tyrosine 3/Tryptophan 5-Monooxygenase Activation Protein, Zeta Polypeptide; 14-3-3 Protein/Cytosolic phospholipase A2; Protein Kinase C Inhibitor Protein-1; Epididymis Secretory Protein Li 93; Epididymis Secretory Protein Li 3; Epididymis Luminal Protein 4; 14-3-3 Protein Zeta/delta; phospholipase A2; 14-3-3-Zeta; HEL-S-93; HEL-S-3; YWHAD;

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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (YWHAZ) Polyclonal Antibody, Unconjugated (orb500615) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Hela (Human) Cell Lysate at 40 ug, A549 (Human) Cell Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Panc-1 (Human) Lysate at 40 ug, Primary: Anti-YWHAZ at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 23 kD, Observed band size: 23 kD.