3'-Hydroxypterostilbene

SKU: orb1220288

Description

3'-Hydroxypterostilbene a natural pterostilbene analogue effectively inhibits the growth of human colon cancer cells with IC50s of 9.0 40.2 and 70.9 μM for COLO 205 HCT-116 and HT-29 cells respectively by inducing apoptosis and autophagy. 3'-Hydroxypterostilbene inhibits the p38MAPK and PI3K/Akt/mTOR/p70S6K pathways and activates the ERK1/2 JNK1/2 MAPK pathways.

Images & Validation

−

Key Properties

−

CAS Number	475231-21-1
MW	272.29
Purity	>98% (HPLC)
Formula	C₁₆H₁₆O₄
SMILES	COc1cc(/C=C/c(cc2)cc(O)c2O)cc(OC)c1
Target	Apoptosis
Solubility	DMSO:26 mg/mL (954.83 mM)

Bioactivity

−

In Vivo

Animal model: 3-4 week old BALB/c nude mice with human colorectal carcinoma COLO 205 tumor xenografts. Dosage: 10 mg/kg. Administration: Intraperitoneal injection; 10 mg/kg once per day for 15 days. Result: Significantly inhibited tumor volume and tumor weight after 15 days treatment compared with the pterostilbene-treated mice, and decreased the protein levels of COX-2, MMP-9, VEGF, cyclin D1 and pro-caspase-3 in mice.

In Vitro

Cell Proliferation Assay Cell line: COLO 205, HCT-116 and HT-29 cell lines. Concentration: 5, 10, 25 and 50 μM. Incubation time: 24 hours. Result: Inhibited cell proliferation of COLO 205, HCT-116 and HT-29 cells with IC50s of 9.0, 40.2 and 70.9 μM, respectively. Apoptosis Analysis Cell line: COLO 205 cells. Concentration: 5-100 μM. Incubation time: 24 hours. Result: Induced apoptosis in human colorectal carcinoma cells. Western blot analysis. Cell line: COLO 205 cells. Concentration: 5-50 μM. Incubation time: 24 hours. Result: Degraded 116 kDa PARP into 85 kDa fragments and induced DFF-45 protein degradation and increased the amounts of LC3B I/II proteins than pterostilbene. Markedly decreased phosphorylation of mTOR, p70S6K (Thr389), PI3K, Akt and p38 MAPK in cells. Cell Autophagy Assay Cell line: COLO 205 cells. Concentration: 25 and 50 μM. Incubation time: 24 hours. Result: Showed an inducing effect on autophagy than pterostilbene in COLO 205 cells.

Storage & Handling

−

Storage	Storage temperature: -20°C. Stability: ≥ 2 years
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

3'-HPT

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

Gene Symbol

Apoptosis

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols