4-Phenylbutyric acid

SKU: orb1301408

Description

4-Phenylbutyric acid is a small molecule functioning as both a histone deacetylase (HDAC) inhibitor and an endoplasmic reticulum stress (ERS) alleviator. It is utilized in research areas including cancer, neurodegeneration, and metabolic disorders, with applications in both cellular and animal models to study protein misfolding and gene expression.

Research Area

Epigenetics & Chromatin, Infectious Disease & Virology, Molecular Biology

Images & Validation

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Key Properties

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CAS Number	1821-12-1
MW	164.2
Purity	99.76%
Formula	C₁₀H₁₂O₂
SMILES	C(CCC(O)=O)C1=CC=CC=C1
Target	Virus Protease,HDAC
Solubility	DMSO:262.5 mg/mL (1598.66 mM);H2O:1.65 mg/mL (10.05 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:12.5 mg/mL (76.13 mM)

Bioactivity

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In Vivo

METHODS: To study the effects on obese mice, 4-Phenylbutyric acid (120 mg/kg) was administered intraperitoneally to KK-Ay mice fed Lieber-DeCarli diet (5% EtOH) once daily for ten days. On day 11, a single dose of EtOH (4 g/kg) was administered by gavage. RESULTS: In an obese mouse model, chronic overdose of EtOH induced massive ER stress-related oxidative stress and liver injury through dysregulation of unfolded protein responses. 4-Phenylbutyric acid ameliorated the liver injury induced by chronic addition of overdose of EtOH by decreasing ER and oxidative stress after overdose of EtOH.

In Vitro

METHODS: ERp57 KO and control cells were treated with 4-Phenylbutyric acid (50 mM) and protein expression levels were detected by Immunoblot analysis. RESULTS: Levels of ER stress marker proteins BiP, Chop and IRE1 were increased in ERp57 KO cells compared to control cells, indicating higher levels of ER stress in the absence of ERp57. 50 mM 4-Phenylbutyric acid treatment was effective in ERp57 KO cells and reduced protein levels of all three ER stress markers. protein levels of all three ER stress markers. In the case of IRE1, ER stress proteins were even reduced below the levels of control cells. METHODS: A549 cells and primary nasal epithelial cells were treated with 4-Phenylbutyric acid (0-20 mM) for 72 h. Cell viability was measured by MTT assay. RESULTS: Cells were examined after treatment with 4-Phenylbutyric acid concentrations ranging from 0-20 mM, and cell survival was not affected below 10 mM.

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Benzenebutyric acid, 4-PBA, 4Phenylbutyric acid, 4-Phenylbutyric acid, 4 Phenylbutyric acid, Inhibitor, HDAC, Histone deacetylases, inhibit, Virus Protease, VirusProtease

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Compound Identifiers

PubChem CID

4775 ↗

Key Properties

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No computed properties available.

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