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4E2RCat

SKU: orb1308720

Description

4E2RCat is a small molecule inhibitor of the eIF4E-eIF4G interaction with an IC50 of 13.5 µM. It effectively suppresses coronavirus replication by reducing viral protein synthesis and the yield of infectious virus, making it a useful tool for studying cap-dependent translation and antiviral mechanisms in vitro.

Research Area

Cell Biology, Infectious Disease & Virology

Images & Validation

Key Properties

CAS Number432499-63-3
MW455.93
Purity98.44% (May vary between batches)
FormulaC22H14ClNO4S2
SMILESOC(=O)c1cc(ccc1Cl)-c1ccc(\C=C2/SC(=S)N(Cc3ccccc3)C2=O)o1
TargetVirus Protease,Autophagy,PERK
SolubilityDMSO:15.5 mg/mL (34 mM)

Bioactivity

Target IC50
eIF4E-eIF4G:13.5 μM
In Vivo
4E2RCat impedes protein synthesis in vivo, and this effect is not due to elevated cell death.
In Vitro
4E2RCat disrupts the interaction between eIF4E (the cap-binding protein) and eIF4G (a large scaffolding protein), thereby inhibiting cap-dependent translation. This action significantly reduces replication of human coronavirus 229E (HCoV-229E), decreasing both the proportion of infected cells and the levels of infectious virus both inside and outside cells. The inhibition of cap-dependent translation by 4E2RCat occurs in a dose-dependent manner, specifically targeting cap-dependent FF translation without affecting EMCV IRES-driven Ren translation. Additionally, 4E2RCat curtails coronavirus replication reliant on dose and duration.

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Virus Protease, 4E2RCat, 4E-2RCat, Autophagy, Inhibitor, eIF4E-Eif4g, eIF4E-eIF4G, Eukaryotic Initiation Factor (eIF), inhibit
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Key Properties

No computed properties available.

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4E2RCat (orb1308720)

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Available Sizes

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25 mg
$ 90.00
50 mg
$ 130.00
1 ml x 10 mM (in DMSO)
$ 160.00
DispatchUsually dispatched within 3-5 working days
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