5-Ethynyl-2'-deoxycytidine (5-EdC)

SKU: orb532418

Description

Ethynyl-labeled deoxycytidine (5-EdC) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) to measure de novo DNA syn-thesis during the S-phase of the cell cycle. 5-EdC is cell-permeabel and incorporates into replicating DNA instead of its natural analog thymidine. The resulting ethynyl-functionalized DNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (Azides of Biotin) for subsequent purification tasks or a fluorescent group (Azides of fluorescent dyes) for subse-quent microscopic imaging.

Images & Validation

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Application Notes	DNA synthesis monitoring. Spectroscopic Propertie: max 291 nm, 8.5 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

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CAS Number	69075-47-4
MW	Theoretical MW: 251.24 g/mol; Detected MW: 251.09 g/mol
Purity	99% (HPLC)
Formula	C₁₁H₁₃N₃O₄
SMILES	n1(cc(c(nc1=O)N)C#C)[C@H]1C[C@@H]([C@H](O1)CO)O

Storage & Handling

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Storage	store at -20 C
Disclaimer	For research use only

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Adler Guerrero Zuniga 1, Timothy J Aikin 1, Connor McKenney 1, Yovel Lendner 2, Alain Phung 2, Paul W Hook 3, Amy Meltzer 3, Winston Timp 3, Sergi Regot Sustained ERK signaling promotes G2 cell cycle exit and primes cells for whole-genome duplication Dev Cell, (2024)

PubMed: 38640927