5-Propargylamino-dCTP - Solution

SKU: orb1733845

Description

Propargylamino-dCTP is recommended for two-step labeling of DNA e.g. by PCR. It is enzymatically incorporated into DNA as substitute for its natural counterpart dCTP. The resulting Amine-functionalized DNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice: to introduce a Biotin group (via NHS Ester of Biotin) for subse-quent purification tasks; to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging.

Images & Validation

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Application Notes	Incorporation into DNA by PCR with Taqpolymerasein-house data. Spectroscopic Propertie: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

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CAS Number	115899-39-3
MW	Theoretical MW: 520.22 g/mol (free acid); Detected MW: 520.02 g/mol (free acid)
Purity	≥ 93% (HPLC)
Formula	C₁₂H₁₉N₄O₁₃P₃
SMILES	NCC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1

Storage & Handling

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Storage	store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Disclaimer	For research use only

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