A-RAF Recombinant Rabbit Monoclonal Antibody

SKU: orb559065

Description

Research Area

Cancer Research, Cell Biology, Kinases, MAPK/ERK Signalling Pathway, Proto-oncogenes, Ras Family, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications	FC, ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200, Flow-Cyt=1:50-100
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	24D1
Immunogen	Recombinant human A-RAF protein (150-300aa)
Target	ARAF
Molecular Weight	68 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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A raf 1; A-Raf; Raf1; A Raf proto oncogene serine/threonine protein kinase; ARAF 1; ARAF; ARaf proto oncogene serine/threonine protein kinase; ARAF1; Oncogene Araf1; Oncogene PKS2; PKS 2; PKS; PKS2; Proto oncogene Pks; RAFA 1; RAFA1; Ras binding protein DA Raf; v raf murine sarcoma 3611 viral oncogene homolog; v raf murine sarcoma 3611 viral oncogene homolog 1; v raf oncogene homolog 1(murine sarcoma 3611 virus).

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A-RAF Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: Human Hela cell lysates, Lane 2: Human MCF-7 cell lysates, Primary: Anti-A-RAF (orb559065) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kDa, Observed band size: 65 kDa.