Alpha-2-Macroglobulin Antibody / A2M

SKU: orb2644474

Description

Alpha-2-Macroglobulin (A2M) is a homotetrameric serum protein consisting of four identical subunits that form dimers through disulfide bonds. Initially, it was characterized as a pan-proteinase inhibitor that was able to bait proteinases into cleaving specific peptide sequences on A2M. This interaction induces a conformational change, thus enabling it to trap the proteinase and further inhibit its activity. Subsequently, A2M has been shown to function as a carrier protein and regulator of cytokines during inflammation.

Images & Validation

−

Item 1 of 3

Tested Applications	IHC-P
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Reactivity	Human
Application Notes	Optimal dilution of the Alpha-2-Macroglobulin antibody should be determined by the researcher.

Key Properties

−

Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2c, kappa
Clone No.	A2M/3621
Immunogen	A portion of amino acids 604-748 from the human protein was used as the immunogen for the Alpha-2-Macroglobulin antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

IHC staining of FFPE human liver with Alpha-2-Macroglobulin antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Alpha-2-Macroglobulin antibody as confirmation of integrity and purity.

Quick Database Links

UniProt

P01023

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

IHC-P

Immunohistochemistry Paraffin

View Protocol

Find More Protocols