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ABCF1 Antibody (C-term)
SKU: orb1928007
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Porcine |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 95926 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−ABC50
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ABCF1 Antibody [orb382536]
WB
Human, Mouse, Rat, Zebrafish
Rabbit
Polyclonal
Unconjugated
200 μl, 100 μl, 30 μl, 50 μl
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Immunohistochemical analysis of paraffin-embedded human kidney tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
All lanes: Anti-ABCF1 Antibody (C-term) at 1:1000 dilution. Lane 1: NCI-H460 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: DU145 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: F9 whole cell lysate. Lane 7: C6 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 96 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ABCF1 Antibody (C-term) (orb1928007)
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