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ACE2 Antibody (Biotin)
SKU: orb1240301
Featured
Description
Research Area
Infectious Disease & Virology
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-ACE2 antibody (biotin) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human ACE2. The immunogen is located within the last 50 amino acids of ACE2. |
| Target | ACE2 |
| Molecular Weight | Predicted: 93kDObserved: 130 kD (7 N-linked glycosylation) |
| Purification | ACE2 Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | ACE2 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−ACE2 Antibody: ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, SARS-CoV receptor, SARS-CoV-2 receptor
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ELISA Validation. Coating Antigen: immunogen peptide, orb1236704, 10 ug/mL, incubate at 4 °C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, orb1240301 or orb1239144, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. orb1240301 was detected by HRP-conjugated streptavidin at 1:5000 and orb1239144 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10000, incubate at RT for 1 hr.
Immunohistochemistry Validation of ACE-2 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti- ACE2 antibody (orb1240301, 5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C, following by streptavidin-HRP conjugate at 20 ug/mL. Counter stained with Hematoxylin.
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IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ACE2 Antibody (Biotin) (orb1240301)
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