Acetyl-Histone H4 (Lys16) Rabbit Polyclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-) HeLa, (+) HeLa + Sodium butyrate (30mM, 4hr), Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 11 kDa, Observed MW: 11 kDa.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:400, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: Rice, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 11 kDa, Observed MW: 11 kDa.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:200, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1172808.

Tissue: Human neuroblastoma, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:200, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172808.