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Acetyl Lysine Recombinant Rabbit Monoclonal Antibody
SKU: orb1974638
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | All |
| Predicted Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 6E11 |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS, Glycerol, rAlbumin. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Acetylated Lysine; Acetyllysine; Ac-lysine; Ac lysine; Ac-K; Ac K; Ac-Lys; Ac Lys; acetyl-Lysine; acetyl Lys; Ac-Lysine;
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Immunoprecipitation of MCF-7 cell extract, IP antibody incubation conditions: orb1974638, 4°C overnight, 1:50 dilution, Incubation conditions for WB first antibody: H3K14ac, room temperature for 2 hours, 1:2000 dilution, Secondary antibody: Goat Anti Rabbit IgG (HRP), Sealing solution and Concentration: 5% NFDM/TBST dilution solution and Concentration: 5% NFDM/TBST, Swim Lane 1: 5% Input, Lane 2: Immunoprecipitation using rabbit monoclonal IgG homotypic control, Lane 3: Immunoprecipitation with orb1974638, Actual molecular weight: 15 kDa, Exposure time: 30 seconds.
Tissue: human liver, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1974638.
Tissue: mouse brain, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1974638.
Tissue: rat liver, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1974638.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Acetyl Lysine Recombinant Rabbit Monoclonal Antibody (orb1974638)
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