Recombinant Acidic Cytokeratin Antibody / Rabbit Monoclonal

SKU: orb2641966

Description

There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile. Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation. The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology. [Wiki]

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Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (2)
Reactivity	Human
Application Notes	Titering of the recombinant Acidic Cytokeratin antibody may be required for optimal performance.1. This antibody will detect cytokeratins -10, -14, -15, -16, -19.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	RMAK1-1
Immunogen	An amino acid sequence common to acidic/type I keratins (1) was used as the immunogen for the recombinant Acidic Cytokeratin antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant Acidic Cytokeratin Antibody / Rabbit Monoclonal

IHC testing of FFPE human lung carcinoma with recombinant Acidic Cytokeratin antibody (clone RMAK1-1). Required HIER: steam sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.

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UniProt

Q7Z794

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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