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ACTA1 Antibody
SKU: orb1939320
Description
Research Area
Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000 |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Gallus, Mouse, Porcine, Rabbit, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,k |
| Molecular Weight | 42051 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−ACTA
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ACTA1 Antibody flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
ACTA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
ACTA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
All lanes: Anti- at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-ACTA1 Antibody at 1:500-1:1000 dilution. Lane 1: MDA-MB231 whole cell lysate. Lane 2: MDA-MB-453 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: mouse liver lysate. Lane 5: CEM whole cell lysate. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42051 Da. Blocking/Dilution buffer: 5% NFDM/TBST.
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UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
ACTA1 Antibody (orb1939320)
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