ACTG1 Antibody (Center)

SKU: orb1930009

Description

Research Area

Immunology & Inflammation, Neuroscience, Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	FC, IF, WB
Dilution Range	WB - 1:1000, FC - 1:10-50, IF - 1:10-50
Reactivity	Human
Predicted Reactivity	Bovine, C. elegans, Drosophila, Gallus, Hamster, Monkey, Mouse, Porcine, Rabbit, Rat, Xenopus, Yeast, Zebrafish

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	41793 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ACTG

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Flow cytometric analysis of K562 cells using ACTG1 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of ACTG1 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of ACTG1 Antibody (Center) in K562, HepG2 cell line lysates (35 ug/lane). ACTG1 (arrow) was detected using the purified Pab.

Western blot analysis of ACTG1 (arrow) using rabbit polyclonal ACTG1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACTG1 gene.

Quick Database Links

UniProt

P63261

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunofluorescence