Actin α3 rabbit pAb

SKU: orb764466

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Description

Rabbit polyclonal antibody to Actin alpha3

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC-P, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human Actin-gamma2. AA range:1-50
Molecular Weight	45
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-ACTG2 antibody, anti-ACTA3 antibody, anti-ACTL3 antibody, anti-ACTSG antibody, anti-Actin antibody, anti- gamma-enteric smooth muscle antibody, anti-Alpha-actin-3 antibody, anti-Gamma-2-actin antibody, anti-Smooth muscle gamma-actin antibody

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Western Blot analysis of various cells using Actin α3 Polyclonal Antibody diluted at 1:2000.

Western Blot analysis of COLO205 cells using Actin α3 Polyclonal Antibody diluted at 1:2000.

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Actin-gamma2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO205 cells, using Actin-gamma2 Antibody. The lane on the right is blocked with the synthesized peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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