Vazyme - Add&Read Human IL8 Quantitative Detection Kit

Interleukin-8 (IL8), also known as CXCL8, is a widely expressed pro-inflammatory member of the CXC family of chemokines, initially identified as a leukocyte chemoattractant. It is secreted by a variety of cell types, including monocytes, macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It acts as a chemoattractant by directing neutrophils to sites of infection, thereby modulating its regulation of the inflammatory response. This kit uses a sandwich method to detect IL8 levels. The kit contains IL8 Standard and two monoclonal antibodies that specifically recognize IL8. One coupled with Eu (donor, Anti-IL8-Eu), and the other coupled with A3 (acceptor, Anti-IL8-A3). When both antibodies bind to IL8 simultaneously, Anti-IL8-Eu and Anti-IL8-A3 come close together, leading to fluorescent resonance energy transfer (FRET). Use 320/340 nm excitation light to excite the fluorescent donor, which emits 620 nm light. The 620 nm light excites the fluorescent receptor, which emits 665 nm light. The concentration of IL8 in the sample is proportional to the FRET signal value (the ratio of fluorescence intensity at 665 nm to 620 nm).