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AKT1 Antibody
SKU: orb379635
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Description
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−Item 1 of 6
| Tested Applications | FC, ICC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IF/ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Monkey, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 1F7E10 |
| Immunogen | Purified recombinant fragment of human AKT1 (AA: 1-150) expressed in E. Coli. |
| Target | Disease |
| Molecular Weight | 55.7kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
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Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of AKT1 antibody
Flow cytometric analysis of Hela cells using AKT1 antibody
Immunofluorescence analysis of HepG2 cells using AKT1 antibody
Western blot analysis of MCF-7 (Lane 1), NIH/3T3 (Lane 2), Hela (Lane 3), COS7 (Lane 4), and C6 (Lane 5) cell lysate using AKT1 antibody
Western blot analysis of HEK293 cell lysate using AKT1 antibody
Western blot analysis of recombinant protein using AKT1 antibody
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ICC
Immunocytochemistry
AKT1 Antibody (orb379635)
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