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Description
Research Area
Cell Biology, Immunology & Inflammation, Immunology & inflammation, Signal Transduction, Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 2
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000, IHC-P: 1:25 |
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Other |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This AKT1/2/3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 278-312 amino acids from the Central region of human AKT1/2/3. Antigen Region: 278-312 aa. |
| Target | AKT1 |
| Molecular Weight | 55686 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, PKB, RAC
Similar Products
−- Item 1 of 2
AKT1/2/3 Antibody (Center) [orb1927101]
IHC-P, WB
Mouse, Other
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl
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Western blot analysis of lysates from PC-3, Hela, rat C6 cell line (from left to right), using AKT1/2/3 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H. prostate carcinoma section using AKT1/2/3 Antibody (Center). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Quick Database Links
Gene Symbol
AKT1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
AKT1/2/3 Antibody (Center) (orb1788467)
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