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Akt1+2+3 Rabbit Polyclonal Antibody
SKU: orb1294323
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | Western Blot 1:1000-1:2000, Immunofluorescence 1:200-1:500 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
| Target | Akt1+2+3 / Pan-AKT |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Disclaimer | For research use only |
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AKT (pan) antibody at 1/1000 dilution, Lysates at 60 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294323 overnight at 4°C.
AKT (pan) antibody at 1/1000 dilution, Lysates at 60 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294323 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294323 anti-AKT (pan) antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294323 anti-AKT (pan) antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Quick Database Links
Gene Symbol
Akt1+2+3 / Pan-AKT
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
Akt1+2+3 Rabbit Polyclonal Antibody (orb1294323)
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