Recombinant Albumin Antibody

SKU: orb2637214

Description

Albumin is a soluble, monomeric protein, which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted form of albumin.

Images & Validation

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Tested Applications	ELISA, IF, IHC-P, WB
Dilution Range	ELISA (order BSA-free format for coating),Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant Albumin antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG
Clone No.	ALB/6413R
Immunogen	Recombinant full-length human ALB protein was used as the immunogen for the recombinant Albumin antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Immunofluorescent staining of human HeP-G2 cells labeled with recombinant Albumin antibody (green, clone ALB/6413R) and RedDot (red).

IHC staining of FFPE human liver carcinoma in colon tissue with recombinant Albumin antibody (clone ALB/6413R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human liver lysates using recombinant Albumin antibody (clone ALB/6413R). Predicted molecular weight ~69 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant Albumin antibody (clone ALB/6413R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P02768

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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