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ALB Antibody
SKU: orb1239194
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Description
Images & Validation
−Item 1 of 7
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Donkey, Equine, Gallus, Porcine, Rabbit |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Gallus |
| Clonality | Polyclonal |
| Isotype | IgY |
| Immunogen | Albumin antibody was raised against a 13 amino acid synthetic peptide near the center of human Albumin.The immunogen is located within amino acids 340 - 390 of Albumin. |
| Target | ALB |
| Molecular Weight | Predicted: 69kD Observed: 69 kD |
| Purification | Albumin Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Albumin Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Albumin Antibody: PRO0883, PRO0903, PRO1341, Serum albumin
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Western Blot Validation in Human Liver. Loading: 10 µg of lysates per lane. Antibodies: Albumin, orb1239194, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Liver. Loading: 10 µg of lysates per lane. Antibodies: Albumin, orb1239194, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Liver. Loading: 10 µg of lysates per lane. Antibodies: Albumin, orb1239194, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of Albumin in Human Liver. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-albumin antibody (orb1239194) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of Albumin in Mouse Liver. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-albumin antibody (orb1239194) at 10 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of Albumin in Rat Liver. Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-albumin antibody (orb1239194) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of Albumin in Mouse Liver. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling albumin with orb1239194 at 20 /mL, followed by goat anti-chicken IgY secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ALB Antibody (orb1239194)
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