Alizarin Complexone

SKU: orb1306798

Description

Alizarin Complexone (Alizarin-3-methyliminodiacetic acid) is a calcium-chelating agent and fluorescent tracer used in biological research for detecting calcium in vitro and in vivo. It also acts as an inhibitor of Rous-associated virus 2 reverse transcriptase.

Research Area

Infectious Disease & Virology

Images & Validation

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Key Properties

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CAS Number	3952-78-1
MW	385.32
Purity	98.97%
Formula	C₁₉H₁₅NO₈
SMILES	O=C1C=2C(C(=O)C=3C1=CC=CC3)=CC(CN(CC(O)=O)CC(O)=O)=C(O)C2O
Target	Reverse Transcriptase
Solubility	DMSO:Slightly soluble

Bioactivity

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Cell Research

I. Solution preparation 1. Mother solution preparation: Dissolve Alizarin Complexone in DMSO to obtain the required mother solution, usually 1-10mM. 2. Working solution preparation: Dilute the mother solution with PBS/DMEM/H2O to the required working solution, usually with a concentration range of 1-10μM. II. Calcium ion detection 1. Add Alizarin Complexone working solution to the cell culture medium and incubate for a certain period of time to allow it to enter the cells and bind to calcium ions in the cells. 2. Use a fluorescence microscope or fluorescence spectrometer to detect the fluorescence signal in the range of 550-600 nm. 3. According to the intensity of the fluorescence signal, calculate the change in calcium ion concentration. II. Reverse transcriptase inhibition experiment: 1. Dissolve Alizarin Complexone in a suitable solvent, usually at a concentration of 1-10 μM. 2. Mix the dissolved Alizarin Complexone with the RCV-2 reverse transcriptase solution. 3. Evaluate the inhibitory effect of Alizarin Complexone on reverse transcriptase activity by measuring reverse transcriptase activity (e.g., by radiolabeling or changes in fluorescence signal).

Storage & Handling

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Storage	keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ReverseTranscriptase, Reverse Transcriptase, RAV-2, a chelating agent, Alizarin Complexone, Alizarin Fluorine Blue, Alizarin-3-methyliminodiacetic acid, antiretroviral activities, calcium tracer, inhibit, Inhibitor

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Compound Identifiers

PubChem CID

65132 ↗

Key Properties

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No computed properties available.

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