Alizarin Red S sodium

SKU: orb1308675

Description

Images & Validation

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Key Properties

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CAS Number	130-22-3
MW	342.26
Purity	90.46%
Formula	C₁₄H₇NaO₇S
SMILES	[Na+].Oc1c(O)c2C(=O)c3ccccc3C(=O)c2cc1S([O-])(=O)=O
Target	Others
Solubility	DMSO:41.67 mg/mL (121.75 mM);H2O:10 mg/mL (29.22 mM)

Bioactivity

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Cell Research

Solution preparation Preparation of mother solution: Prepare a 2% Alizarin Red S sodium aqueous solution. Preparation of working solution: Use ammonium hydroxide to adjust the pH to 4.2, filter the solution with a 0.45 μm microfilter and store it in an amber bottle and re-filter the solution with a 0.22 μm microfilter before use. I. Detection of osteoblast differentiation by Alizarin Red S sodium Operation steps 1. Induce osteoblast differentiation: MC3T3-E1 cells were seeded on four-well plates at 1×105 cells per well and cultured in α-MEM (without ascorbic acid) containing 10% fetal bovine serum. Osteoblast differentiation was induced with osteogenic medium (OSM) containing ascorbic acid (50 μg/mL) and β-glycerophospholipids (10 mM). 2. Staining: MC3T3-E1 cells were washed with 1×PBS, fixed with 70% ethanol at room temperature for 10 min, rinsed with distilled water, and stained with Alizarin Red S for 10 min to detect calcium deposition in the matrix. 3. Data analysis: The area of the red area is proportional to osteoblast differentiation. 2. Screening test of calcium compounds in synovial fluid by Alizarin Red S sodium 1. Synovial fluid preparation: Aspirate the synovial fluid into a disposable syringe, and then transfer the fluid to a clean test tube containing sodium heparin anticoagulant; 2. Staining: Aspirate a drop of synovial fluid from the bottom of the test tube, place it on a clean slide, mix it with a drop of 2% Alizarin Red S, and cover it with a coverslip; c3 Within three minutes after mixing, observe it under an ordinary optical microscope, and grade the degree of orange-red staining density observed in each high-power field of view. The area of the red area is proportional to the content of calcium compounds.

Storage & Handling

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Storage	keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ARS, ARS sodium, Alizarin Red S, Alizarin Red S sodium, Inhibitor, inhibit

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Compound Identifiers

PubChem CID

3955344 ↗

Key Properties

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No computed properties available.

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