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Aloperine

SKU: orb1300821

Description

Aloperine is a natural alkaloid with documented anti-inflammatory, antibacterial, antiviral, and anti-tumor activities. It is utilized in pharmacological research for studying these mechanisms in both cellular (in vitro) and animal (in vivo) experimental models.

Research Area

Cell Biology, Infectious Disease & Virology, Protein Biochemistry

Images & Validation

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Aloperine

Key Properties

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CAS Number56293-29-9
MW232.36
Purity99.88% (May vary between batches)
FormulaC15H24N2
SMILES[C@]12([C@]3(C(=C[C@@](C1)([C@@]4(N(C2)CCCC4)[H])[H])CCCN3)[H])[H]
TargetVirus Protease,Antibiotic,HIV Protease,Apoptosis,Autophagy
Solubility10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (8.61 mM);Ethanol:46 mg/mL (197.97 mM);DMSO:55 mg/mL (236.7 mM)

Bioactivity

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In Vivo
Contrary to its effect on leukemia cells, Aloperine at a concentration of up to 1 mM did not significantly reduce the viability of normal PBMNCs after 72 hours. A 20 μM dose of Aloperine administered for 48 hours was observed to induce apoptosis and autophagy in HL-60 cells in a dose-dependent manner. The strongest cytotoxic effect of Aloperine on HL-60 cells was observed at 72 hours, with an inhibition rate of 94.1%.
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In Vitro
When topically applied to NC/Nga mice, Aloperine significantly and dose-dependently reduced 2,4-dinitrofluorobenzene (DNFB)-induced dermatitis as indicated by dermatitis scores and ear thickness on days 13 and 14. Additionally, a 1% topical application of Aloperine on BALB/c mice inhibited the increase in ear swelling and redness triggered by DNFB, and markedly reduced the upregulation of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6) mRNA and protein levels in ear biopsy homogenates. Aloperine dose-dependently diminished DNFB-induced lymphocyte and eosinophil infiltration, as well as mast cell infiltration in treated regions. Histological examination of ear tissues from treated NC/Nga mice also showed a clear dose-dependent decrease in the levels of cytokines TNF-α, IL-1β, and IL-6. Furthermore, Aloperine significantly lowered the elevated levels of IL-4, IL-13, and IFN-γ induced by DNFB, while dose-dependently increasing IL-10 levels, indicating its effective modulation of inflammatory responses.
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Cell Research
Cells are seeded in 96-well plates in 100-μL culture medium. After incubation of 4 hours for leukaemia cells and of 24 hours for solid cancer cells, experimental media containing either excipient control or Aloperine are added to appropriate wells. Five concentrations of Aloperine for 48-hour treatment are used to determine the in vitro IC50 growth inhibitory values of Aloperine in cancer cells. After incubation, 10 μL of MTT solution (5 mg/mL) is added to each well. The plates are then incubated for 4 hours at 37 °C. Intracellular formazan crystals are dissolved by addition of 100 μL of isopropanol-HCI-SDS solution to each well. After an overnight incubation at 37 °C, the optical density of the samples is determined at 570 nm. DNA fragmentation is analysed after the extraction of DNA from cells exposed to the indicated doses of Aloperine for 48 hours using apoptotic DNA ladder kit. For autophagy detection, cells are collected and incubated with PBS containing 5 μM acridine orange for 15 minutes. The acridine orange is removed and the cells are resuspended in 100 μL of PBS. Fluorescent micrographs are obtained with an inverted fluorescent microscope. Autophagy is quantified based on the mean number of cells displaying intense red staining for three fields (containing at least 50 cells per field) for each experimental condition.(Only for Reference)
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Storage & Handling

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StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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inhibit, Inhibitor, Filovirus, Human immunodeficiency virus, HIV Protease, HIV, HIVProtease, Apoptosis, Antibiotic, Autophagy, Aloperine, VirusProtease, Virus Protease

Similar Products

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  • Aloperine

    Aloperine [orb1222037]

    >98% (HPLC)

    56293-29-9

    232.36

    C15H24N2

    1 g, 500 mg, 200 mg, 100 mg, 50 mg
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID
162147 ↗

Key Properties

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No computed properties available.

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Aloperine (orb1300821)

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% DMSO +
%+
% Tween 80 +
%

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1 ml x 10 mM (in DMSO)
$ 70.00
100 mg
$ 80.00
500 mg
$ 130.00
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