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Description
Research Area
Metabolism Research
Images & Validation
−Item 1 of 5
| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Dilution Range | IF - 1:100, WB - 1:1000, IHC-P - 1:10-50, FC - 1:10-50 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This ALOX12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 618-650 amino acids from the C-terminal region of human ALOX12. Antigen Region: 618-650 aa. |
| Target | ALOX12 (HGNC:429) |
| Molecular Weight | 75694 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Arachidonate 12-lipoxygenase, 12S-type, 12S-LOX, 12S-lipoxygenase, Lipoxin synthase 12-LO, 332-, Platelet-type lipoxygenase 12, ALOX12, 12LO, LOG12
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ALOX12 Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-ALOX12 Antibody (C-term) at 1:2000 dilution + Mouse lung whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 76 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes: Anti-ALOX12 Antibody (C-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Rat spleen whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 76 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of A549 cells, using ALOX12 Antibody (C-term). Diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000688.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
ALOX12 Antibody (C-term) (orb1928230)
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