AMACR Rabbit Polyclonal Antibody

SKU: orb10075

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Description

Research Area

Cancer Research, Cell Biology, Lipid Metabolism, Lipids and Lipoproteins Metabolism, Metabolism, Mitochondrial Markers, Oxidative Stress, Proto-oncogenes, Tumor Biology

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution Range	WB=1:500-2000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Human, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Mouse AMACR protein
Target	AMACR
Molecular Weight	42 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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AMACR_HUMAN; alpha-methylacyl-CoA racemase; EC:5.1.99.4; 2-methylacyl-CoA racemase; RM; RACE; CBAS4; P504S; AMACRD;

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (AMACR) Polyclonal Antibody, Unconjugated (orb10075) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Mouse Kidney tissue lysates, Lane 3: Rat Liver tissue lysates, Lane 4: Rat Kidney tissue lysates, Primary: Anti-AMACR (orb10075) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kDa, Observed band size: 38 kDa.

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Gene Symbol

AMACR

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Protocol Information

Western Blot (IB, immunoblot)

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