Aminoallyl-dUTP-5/6-TAMRA

SKU: orb64173

Description

Aminoallyl-dUTP-5/6-TAMRA is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-5/6-TAMRA/dTTP ratio for Nick Translation: 35% Aminoallyl-dUTP-5/6-TAMRA/65% dTTP.

Images & Validation

−

Application Notes	Incorporation into DNA/cDNA by Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λabs 545 nm, λem 575 nm, ε 90.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

−

MW	Theoretical MW: 935.66 g/mol (free acid); Detected MW: 935.16 g/mol (free acid)
Purity	≥ 95% (HPLC)
Formula	C₃₇H₄₀N₅O₁₈P₃
SMILES	C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)c1cc(c(cc1)C(=O)[O-])c1c2c(oc3c1ccc(c3)N(C)C)cc(=[N+](C)C)cc2.C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)c1ccc(c(c1)C(=O)[O-])c1c2c(oc3c1ccc(c3)N(C)C)cc(=[N+](C)C)cc2

Storage & Handling

−

Storage	store at -20 °C
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID

176416309 ↗

Key Properties

−

No computed properties available.

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols