Aminoallyl-dUTP-XX-ATTO-594

SKU: orb611221

Description

Aminoallyl-dUTP-XX-ATTO594 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-XX-ATTO594/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-ATTO594/70-50% dTTP.

Images & Validation

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Application Notes	Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 602 nm, λem 626 nm, ε 120.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

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MW	Theoretical MW: 1537.48 g/mol (free acid); Detected MW: 1536.44 g/mol (free acid)
Purity	≥ 95% (HPLC)
Formula	C₆₅H₈₇N₈O₂₅P₃S₂
SMILES	C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCN(C(=O)c1c(cccc1)C1=c2c(Oc3c1cc1c(c3)N(C(C=C1CS(=O)(=O)O)(C)C)CC)cc1=[N+](C(C=C(c1c2)CS(=O)(=O)[O-])(C)C)CC)C

Storage & Handling

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Storage	store at -20 °C
Disclaimer	For research use only

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