Aminoallyl-dUTP-XX-ATTO-643

SKU: orb611222

Description

Aminoallyl-dUTP-XX-643 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. ATTO 643 is a hydrophilic dye with excellent photostability compared to fluorescein. Recommended Aminoallyl-dUTP-XX-643/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-XX-643/80-70% dTTP.

Images & Validation

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Application Notes	Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 643 nm, λem 665 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

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MW	Theoretical MW: 1567.90 g/mol (free acid)
Purity	≥ 95% (HPLC)
Formula	C₂₄H₄₁N₅O₁₆P₃-ATTO 643
SMILES	C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCCCCNC(=O)CCCCCN*

Storage & Handling

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Storage	store at -20 °C
Disclaimer	For research use only

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