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Amyloid Precursor Protein Rabbit Polyclonal Antibody
SKU: orb10119
Featured
Description
Research Area
DNA / RNA Binding, DNA and RNA Research, RNA Processing
Images & Validation
−Item 1 of 2
| Tested Applications | ICC, IF, IHC-Fr, IHC-P |
|---|---|
| Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
| Reactivity | Human, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from of human APP (381-480/770aa) |
| Target | APP |
| Molecular Weight | 72-83 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Soluble APP-alpha; Soluble APP-beta; A4 amyloid protein; A4; AAA; ABETA; ABPP; AD 1; AD1; Alzheimer disease 1; Alzheimer disease; Alzheimer's disease amyloid protein; Amyloid beta(A4) precursor protein; Amyloid beta A4 protein; Amyloid beta A4 protein precursor isoform b; Amyloid beta A4 protein precursor isoform c; Amyloid beta A4 protein precursor isoform a; Amyloid beta A4 protein precursor isoform b; Amyloid beta A4 protein precursor isoform c; Amyloid beta protein; Amyloid beta-peptide; Amyloid of aging and alzheimer disease; APP; APP I; APPI; beta amyloid peptide; Cerebral vascular amyloid peptide; CTFgamma; CVAP; Human mRNA for amyloid A4 precursor of Alzheimer's disease; PN 2; PN II; PN2; PreA4; Protease nexin II; A4_HUMAN.
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![APP antibody [C2C3], C-term](/images/pub/media/catalog/product/a/p/app-antibody_orb555949_ifa_1.jpg)
![APP antibody [C2C3], C-term](/images/pub/media/catalog/product/a/p/app-antibody_orb555949_ihc_1.jpg)
![APP antibody [C2C3], C-term](/images/pub/media/catalog/product/a/p/app-antibody_orb555949_ihc-p_1.jpg)
![APP antibody [C2C3], C-term](/images/pub/media/catalog/product/a/p/app-antibody_orb555949_wb_1.jpg)
![APP antibody [C2C3], C-term](/images/pub/media/catalog/product/a/p/app-antibody_orb555949_wb_2.jpg)
![APP antibody [C2C3], C-term](/images/pub/media/catalog/product/a/p/app-antibody_orb555949_wb_3.jpg)
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Tissue/cell: MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Amyloid Precursor Protein) polyclonal Antibody, Unconjugated (orb10119) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Amyloid Precursor Protein Polyclonal Antibody, Unconjugated (orb10119) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Quick Database Links
Gene Symbol
APP
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
Amyloid Precursor Protein Rabbit Polyclonal Antibody (orb10119)
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