ANGPT2 Antibody (C-term)

SKU: orb1939167

This product can be custom made

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cardiovascular Research

Images & Validation

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Item 1 of 4

Tested Applications	FC, IHC-P, WB
Dilution Range	WB - 1:2000, IHC-P - 1:50-100, FC - 1:25
Reactivity	Human, Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	BTD39901
Immunogen	This ANGPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 404-432 amino acids from the C-terminal region of human ANGPT2. Antigen Region: 404-432 aa.
Target	ANGPT2
Molecular Weight	56919 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Angiopoietin-2, ANG-2, ANGPT2

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

ANGPT2 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35 ug/lane). This demonstrates the ANGPT2 antibody detected the ANGPT2 protein (arrow).

ANGPT2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ANGPT2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Anti-ANGPT2 Antibody (C-term) at 1:2000 dilution + human liver lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing A549 cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.