ADAMTS13 Rabbit Polyclonal Antibody

Sandwich ELISA - Recombinant human ADAMTS13 protein standard curve. Use in combination with reagents from Human ADAMTS13 ELISA Kit EZ-Set (DIY Antibody Pairs).

Western blot analysis of ADAMTS13 using anti-ADAMTS13 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: rat brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS13 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADAMTS13 at approximately 154 KD. The expected band size for ADAMTS13 is at 154 KD.

Western blot analysis of ADAMTS13 using anti-ADAMTS13 antibody. Western blot analysis of ADAMTS13 expression in human HepG2 whole cell lysates 50 ug, ADAMTS13 at 154 KD under non-reducing conditions and 200 kD under reducing conditions were detected using rabbit anti-ADAMTS13 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.