Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human ADRB1, which shares 81.2% amino acid (aa) sequence identity with mouse and rat ADRB1.
Target	Beta-1 adrenergic receptor
Molecular Weight	51 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Concentration	500 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ADRB1; ADRB1R; adrenergic; beta 1; receptor; B1AR; Beta 1 adrenergic receptor; Beta 1 adrenoceptor; Beta 1 adrenoreceptor; BETA1AR; RHR

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Western blot analysis of ADRB1 using anti-ADRB1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat NRK whole cell lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRB1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADRB1 at approximately 51 kDa. The expected band size for ADRB1 is at 51 kDa.

Quick Database Links

Gene Symbol

Beta-1 adrenergic receptor

UniProt

P08588

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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