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AF3P21 Antibody
SKU: orb234753
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution range | WB: 1:500-1000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AF3P21. The exact sequence is proprietary. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−AF3P21; SPIN90; NCK-interacting protein with SH3 domain; 54 kDa VacA-interacting protein; 54 kDa vimentin-interacting protein; VIP54; 90 kDa SH3 protein interacting with Nck; AF3p21; Dia-interacting protein 1; DIP-1; Diaphanous protein-interacting protein; SH3 adapter protein SPIN90; WASP-interacting SH3-domain protein; WISH; Wiskott-Aldrich syndrome protein-interacting protein
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Western blot analysis of AF3P21 expression in HEK293T (A), Raw264.7 (B), H9C2 (C) whole cell lysates. (Predicted band size: 78 kD; Observed band size: 78 kD)
Immunohistochemical analysis of AF3P21 staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
AF3P21 Antibody (orb234753)
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