Alpha-adducin Rabbit Polyclonal Antibody

SKU: orb304678

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Description

The Alpha-adducin Antibody is suitable for IF, IHC, IP, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Alpha-adducin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

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Item 1 of 7

Tested Applications	IF, IHC, IP, WB
Dilution Range	WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500, IP: 1:10-100
Reactivity	Bovine, Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Alpha-adducin. The exact sequence is proprietary.
Target	ADD1; ADD2
Molecular Weight	80 kDa, 120 kDa
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ADD1; ADDA; Alpha-adducin; Erythrocyte adducin subunit alpha; ADD2; ADDB; Beta-adducin; Erythrocyte adducin subunit beta

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Alpha-adducin Rabbit Polyclonal Antibody

Immunofluorescent analysis of Alpha-adducin staining in HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Immunohistochemical analysis of paraffin-embedded human breast cancer using Alpha-adducin Antibody orb304678 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded mosue spleen using Alpha-adducin Antibody orb304678 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat liver using Alpha-adducin Antibody orb304678 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat lung using Alpha-adducin Antibody orb304678 with dilution at 1:100.

Western blot analysis of mouse brain (1), rat brain (2) using Alpha-adducin Antibody orb304678 with dilution at 1:1000.

Quick Database Links

Gene Symbol

ADD1; ADD2

UniProt

Q63028, P35611, P35612, Q9QYC0, Q9QYB8, Q05764

NCBI Gene ID

24170, 11519, 24171, 118, 11518, 119

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

Immunofluorescence

Immunoprecipitation