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ANO1 Antibody
SKU: orb705623
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution range | WB: 1:500-1:2000, IF: 1:50-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein of human ANO1. The exact sequence is proprietary. |
| Target | ANO1 |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−Anti-DOG1 antibody, anti-ORAOV2 antibody, anti-TAOS2 antibody, anti-TMEM16A antibody, anti-Anoctamin-1 antibody, anti-Discovered on gastrointestinal stromal tumors protein 1 antibody, anti-Oral cancer overexpressed protein 2 antibody, anti-Transmembrane protein 16A antibody, anti-Tumor-amplified and overexpressed sequence 2 antibody
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Western blot analysis of ANO1 expression in LO2 (A), HeLa (B), mouse lung (C), rat skeletal muscle (D) whole cell lysates. (Predicted band size: 74; 97; 114 kD; Observed band size: 114 kD)
Immunofluorescent analysis of ANO1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ANO1 Antibody (orb705623)
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