ARID1A Rabbit Polyclonal Antibody

SKU: orb316548

Description

Anti-ARID1A Antibody. Tested in WB applications. This antibody reacts with Human.

Research Area

Cancer Biology, Epigenetics & Chromatin, Signal Transduction

Images & Validation

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Tested Applications	FC, ICC, IF, WB
Dilution Range	Western blot, 0.1-0.5μg/ml, Human, Mouse Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry(Fixed), 1-3 μg/1x10^6 cells, Human
Reactivity	Human, Mouse

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	A synthetic peptide corresponding to a sequence in the middle region of human ARID1A, identical to the related mouse sequence.
Target	AT-rich interactive domain-containing protein 1A
Molecular Weight	250-270 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Disclaimer	For research use only

Alternative Names

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AT-rich interactive domain-containing protein 1A; ARID domain-containing protein 1A; B120; BRG1-associated factor 250; BAF250; BRG1-associated factor 250a; BAF250A; Osa homolog 1; hOSA1; SWI-like protein; SWI/SNF complex protein p270; SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1; hELD; ARID1A; BAF250, BAF250A, C1orf4, OSA1, SMARCF1

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Western blot analysis of ARID1A using anti-ARID1A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: SW620 Whole Cell Lysate at 40 ug. Lane 2: HEPG2 Whole Cell Lysate at 40 ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARID1A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ARID1A at approximately 242 kDa. The expected band size for ARID1A is at 242 kDa.

Quick Database Links

Gene Symbol

AT-rich interactive domain-containing protein 1A

UniProt

O14497

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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