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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, FC |
|---|---|
| Dilution Range | ELISA 1/5000-10000|Flow Cyt 1/100 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | BFO19 |
| Target | B7-H2 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ICOSLG, B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, LICOS, ICOS ligand
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ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-H2 protein, mFc-His tagged protein orb689390 can bind Rabbit anti-B7-H2 monoclonal antibody (clone: DM97) in a linear range of 3.2-80 ng/ml.
Flow cytometry analysis with Anti-B7-H2 (DM97) on Expi293 cells transfected with human B7-H2 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Flow cytometry analysis of antigen binding of rabbit anti-human B7-H2 mAb (orb1174071). (A) orb1174071 does not bind to Jurkat cells that do not express B7-H2. (B) A clear peak shift of orb1174071 was seen compared to the control when incubated with B7-H2-expressing Siha cells, indicating strong binding of orb1174071 to B7-H2. Antibodies were incubated at 5 μg/mL.
Quick Database Links
Gene Symbol
B7-H2
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
B7-H2 Antibody (orb1174071)
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