B7-H5 Biosimilar Antibody

SKU: orb1291214

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Description

Anti-B7-H5(onvatilimab biosimilar) mAb

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, FC
Dilution range	ELISA 1:5000-10000 Flow Cyt 1:100
Reactivity	Human
Application Notes	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

Key Properties

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Antibody Type	Biosimilar Antibody
Host	Human
Clonality	Monoclonal
Isotype	IgG1
Target	B7-H5
Purification	Purified from cell culture supernatant by affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Powder
Disclaimer	For research use only

Alternative Names

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VISTAVSIRSisp-1C10orf54DD1alphaDies1GI24PD-1HPP2135

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ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human B7-H5 Protein, hFc Tag (orb1173726) can bind Anti-B7-H5 Neutralizing antibody (orb1291214) in a linear range of 2.44–625.00 ng/ml. In order to specifically detect orb1291214, mouse anti-human Fab-specific antibody was used as detection antibody.

Flow cytometry analysis with 1 μg/mL Anti-B7-H5 (onvatilimab biosimilar) mAb (orb1291214) on Expi293 cells transfected with Human B7-H5 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Flow cytometry analysis of antigen binding of anti-human B7-H5 mAb (orb1291214). (A) orb1291214 does not bind to 293T cells that do not express B7-H5. (B) A clear peak shift of orb1291214 was seen compared to the control when incubated with B7-H5-expressing THP-1 cells, indicating strong binding of orb1291214 to B7-H5. Antibodies were incubated at 5 μg/mL.

Quick Database Links

Gene Symbol

B7-H5

UniProt

Q9H7M9

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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