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betaIII-Tubulin Antibody (FITC)
SKU: orb44545
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 2
| Tested Applications | FC, ICC |
|---|---|
| Reactivity | Canine, Human, Mouse, Porcine, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | TU-20 |
| Immunogen | Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide. |
| Target | betaIII-Tubulin |
| Purification | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Conjugation | FITC |
Storage & Handling
−| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TUBB3
Quality Guarantee
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Separation of MCF-7 cells stained using anti-human betaIII-Tubulin (TU-20) FITC antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from MCF-7 cells stained using mouse IgG1 isotype control (MOPC-21) FITC antibody (concentration in sample 15 μg/ml, same as betaIII-Tubulin FITC concentration, black-dashed) in flow cytometry analysis (intracellular staining) of MCF-7 cell suspension.
Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 FITC, green, 1 μg/ml). Nuclei were stained with DAPI (blue).
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Protocol Information
FC
Flow Cytometry
ICC
Immunocytochemistry
betaIII-Tubulin Antibody (FITC) (orb44545)
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