BRCA1 Rabbit Polyclonal Antibody

SKU: orb180713

Description

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Molecular Biology, Protein Biochemistry

Images & Validation

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Item 1 of 8

Tested Applications	FC, IHC, WB
Dilution Range	Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
Reactivity	Human, Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E.coli-derived human BRCA1 recombinant protein (Position: E1661-Y1863). Human BRCA1 shares 65% and 66% amino acid (aa) sequences identity with mouse and rat BRCA1, respectively.
Target	Breast cancer type 1 susceptibility protein
Molecular Weight	290 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Breast cancer type 1 susceptibility protein; 6.3.2.-; RING finger protein 53; BRCA1; RNF53

Phospho-RAD51 (Thr309) Rabbit Polyclonal Antibody [orb4742]

Applications:	FC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Gallus, Porcine, Rabbit, Rat, Sheep
Reactivity:	Human, Mouse
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 200 μl, 50 μl, 100 μl

Item 1 of 5
BARD1 Rabbit Polyclonal Antibody [orb100312]
Applications:
FC, IF, IHC-Fr, IHC-P
Predicted Reactivity:
Bovine, Canine, Equine, Gallus, Porcine
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 50 μl, 100 μl, 200 μl

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Flow Cytometry analysis of HepG2 cells using anti-BRCA1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of BRCA1 using anti-BRCA1 antibody.Lane 1:human HeLa cell;2:human T-47D cell;3:human Raji cell.

IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of human tonsil tissue.

IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue.

IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of human thyroid cancer tissue.

IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue.

IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of mouse brain tissue.

IHC analysis of BRCA1 using anti-BRCA1 antibody. BRCA1 was detected in a paraffin-embedded section of rat brain tissue.

Quick Database Links

Gene Symbol

Breast cancer type 1 susceptibility protein

UniProt

P38398

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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